8DMR
Legionella macrodomain effector MavL R370A in complex with ADP-ribose
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-02-20 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9792 |
Spacegroup name | P 41 |
Unit cell lengths | 95.557, 95.557, 54.046 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.210 - 1.860 |
R-factor | 0.152 |
Rwork | 0.151 |
R-free | 0.17980 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6omi |
RMSD bond length | 0.012 |
RMSD bond angle | 1.219 |
Data reduction software | XDS |
Data scaling software | XDS |
Phasing software | PHASER |
Refinement software | PHENIX (1.18.2_3874) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.210 | 1.927 |
High resolution limit [Å] | 1.860 | 1.860 |
Number of reflections | 41115 | 4087 |
<I/σ(I)> | 23.61 | |
Completeness [%] | 99.7 | |
Redundancy | 4.6 | |
CC(1/2) | 0.998 | 0.781 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20% PEG 3350, 0.2 M ammonium sulfate |