8DLD
Crystal structure of chalcone-isomerase like protein from Physcomitrella patens (PpCHIL-A)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.1 |
| Synchrotron site | ALS |
| Beamline | 8.2.1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-24 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 62.550, 49.880, 76.820 |
| Unit cell angles | 90.00, 110.87, 90.00 |
Refinement procedure
| Resolution | 37.300 - 1.850 |
| R-factor | 0.189 |
| Rwork | 0.185 |
| R-free | 0.22440 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4dok |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.098 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.940 | 1.950 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.102 | 0.667 |
| Rpim | 0.049 | 0.326 |
| Number of reflections | 18703 | 2696 |
| <I/σ(I)> | 8.1 | 2.1 |
| Completeness [%] | 98.6 | |
| Redundancy | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 277.15 | 30% PEG 4K and 0.2 M ammonium sulfate |
