8DKF

Antibody DH1030.1 Fab fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-23
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1
Spacegroup name	P 21 21 21
Unit cell lengths	81.190, 103.120, 109.846
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.520 - 1.790
R-factor	0.2397
Rwork	0.239
R-free	0.27370
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6pdu
RMSD bond length	0.008
RMSD bond angle	0.888
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.830
High resolution limit [Å]	1.790	1.790
Rmerge	0.121	0.846
Rmeas	0.129	0.902
Rpim	0.043	0.308
Number of reflections	85926	4207
<I/σ(I)>	36.67	1.93
Completeness [%]	99.5	99.2
Redundancy	9.7	8.3
CC(1/2)	0.981	0.930

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		295	30% (v/v) MPD 100 mM Sodium acetate/ Acetic acid pH 4.5 25% (w/v) PEG 1500