8DK9
Sliding-clamp-DinX peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-07-08 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1 |
Spacegroup name | P 1 |
Unit cell lengths | 71.072, 73.944, 81.343 |
Unit cell angles | 82.77, 77.57, 87.75 |
Refinement procedure
Resolution | 47.350 - 2.500 |
R-factor | 0.2126 |
Rwork | 0.211 |
R-free | 0.26440 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 8dj6 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.481 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.350 | 2.540 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.068 | 0.329 |
Rpim | 0.068 | 0.329 |
Number of reflections | 52753 | 2313 |
<I/σ(I)> | 6.66 | |
Completeness [%] | 94.5 | |
Redundancy | 1.7 | |
CC(1/2) | 0.996 | 0.634 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.1 M Hepes ph 7.5, 0.1M lithium sulphate, 0.1 M NaCl, 20%PEG 3350 and 10% 1,6-Hexanediol |