8DK6

Structure of hepatitis C virus envelope N-terminal truncated glycoprotein 2 (E2) (residues 456-713) from J6 genotype

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-12-08
Detector	DECTRIS EIGER2 XE 16M
Wavelength(s)	0.979
Spacegroup name	P 2 21 21
Unit cell lengths	48.970, 125.540, 157.320
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	48.390 - 2.450
R-factor	0.2046
Rwork	0.203
R-free	0.23180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4web
RMSD bond length	0.003
RMSD bond angle	0.682
Data reduction software	iMOSFLM (7.0.077)
Data scaling software	SCALA (7.0.077)
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.970	2.580
High resolution limit [Å]	2.450	2.450
Rmerge	0.109	0.582
Rpim	0.062	0.334
Number of reflections	35577	5166
<I/σ(I)>	8.8	3.1
Completeness [%]	97.8	99.1
Redundancy	4	4.2
CC(1/2)	0.991	0.571

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.3	293.15	0.2M sodium citrate tribasic dihydrate, 20% w/v PEG 3350, pH 8.3, Cryoprotectant used 30% Ethylene glycol