8DJQ
Sliding-clamp-DnaE1 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-07-08 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 66.810, 148.252, 81.952 |
Unit cell angles | 90.00, 92.18, 90.00 |
Refinement procedure
Resolution | 19.870 - 2.800 |
R-factor | 0.2281 |
Rwork | 0.226 |
R-free | 0.28490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 8dj6 |
RMSD bond length | 0.002 |
RMSD bond angle | 0.479 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 48.062 | 2.850 |
High resolution limit [Å] | 2.800 | 2.800 |
Rmerge | 0.153 | |
Rpim | 0.098 | |
Number of reflections | 38932 | 1673 |
<I/σ(I)> | 4.75 | |
Completeness [%] | 99.5 | |
Redundancy | 3.3 | |
CC(1/2) | 0.967 | 0.967 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 298 | 0.5M Ammonium sulphate, 0.1 M sodium acetate tribasic pH 5.6 |