8DJI

Ternary complex of SUMO1 with the SIM of PML and zinc

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	298
Detector technology	PIXEL
Collection date	2019-03-20
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.977
Spacegroup name	P 21 21 21
Unit cell lengths	36.995, 47.156, 63.777
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.000 - 1.970
R-factor	0.2336
Rwork	0.231
R-free	0.25780
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6v7p
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.000	2.044
High resolution limit [Å]	1.970	1.970
Number of reflections	14192	637
<I/σ(I)>	5.34
Completeness [%]	96.6
Redundancy	5.5
CC(1/2)	0.982	0.263

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	100 mM HEPES pH 7.0, 19 to 31 % (w/v) PEG 3350, and 1-5 mM zinc chloride