8DIN
The complex structure between human IgG1 Fc and its high affinity receptor FcgRI H174R variant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 22-ID |
| Synchrotron site | APS |
| Beamline | 22-ID |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2019-06-03 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 1 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 203.997, 88.804, 55.925 |
| Unit cell angles | 90.00, 98.05, 90.00 |
Refinement procedure
| Resolution | 36.770 - 2.500 |
| R-factor | 0.1793 |
| Rwork | 0.177 |
| R-free | 0.22310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4x4m |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.530 |
| High resolution limit [Å] | 2.480 | 2.480 |
| Rmerge | 0.118 | 0.579 |
| Number of reflections | 34744 | 2137 |
| <I/σ(I)> | 14.1 | |
| Completeness [%] | 98.2 | 97.8 |
| Redundancy | 5.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20% PEG3350, 0.2M Magnesium formate |
