8DI1
Bfo2294: Tannerella forsythia 2-Keto-3-deoxy-6-phosphogluconate aldolase (KDPG) and 4-Hydroxy-2-oxoglutarate aldolase (KHG)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08ID-1 |
Synchrotron site | CLSI |
Beamline | 08ID-1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2017-07-12 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.97948 |
Spacegroup name | P 2 3 |
Unit cell lengths | 98.150, 98.150, 98.150 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 34.700 - 2.200 |
R-factor | 0.1812 |
Rwork | 0.179 |
R-free | 0.22090 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1wa3 |
RMSD bond length | 0.003 |
RMSD bond angle | 0.606 |
Data reduction software | autoPROC |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.890 | 2.320 |
High resolution limit [Å] | 2.200 | 2.200 |
Rpim | 0.041 | 0.216 |
Number of reflections | 16309 | 2348 |
<I/σ(I)> | 12.4 | 3.4 |
Completeness [%] | 100.0 | 100 |
Redundancy | 11 | 7.4 |
CC(1/2) | 0.995 | 0.878 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 294 | 40% (v/v) Tacsimate pH 7.0 and 11% (v/v) Tacsimate pH 9.0 and 6.5% (v/v) glycerol as the crystallization solution |