8DHR
An ester mutant of SfGFP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 4.2.2 |
Synchrotron site | ALS |
Beamline | 4.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-06-01 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.0 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 129.917, 37.492, 91.848 |
Unit cell angles | 90.00, 106.31, 90.00 |
Refinement procedure
Resolution | 44.080 - 1.750 |
R-factor | 0.1808 |
Rwork | 0.181 |
R-free | 0.18830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2b3p |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.1_4122) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.150 | 1.780 |
High resolution limit [Å] | 1.750 | 1.750 |
Number of reflections | 42753 | 2475 |
<I/σ(I)> | 15.5 | 2.4 |
Completeness [%] | 99.3 | |
Redundancy | 3.5 | |
CC(1/2) | 0.990 | 0.840 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 291 | 50 mM Hepes, pH 7.5 and 25% Peg4000 |