8DHK
Crystal structure of human Sulfide Quinone Oxidoreductase K207E
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-D |
Synchrotron site | APS |
Beamline | 21-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-02-16 |
Detector | DECTRIS EIGER X 9M |
Wavelength(s) | 0.9762 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 76.793, 111.298, 134.105 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 33.405 - 2.300 |
R-factor | 0.1987 |
Rwork | 0.197 |
R-free | 0.23680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6oi6 |
Data reduction software | DIALS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 45.100 | 2.370 |
High resolution limit [Å] | 2.300 | 2.300 |
Rmerge | 0.154 | 1.404 |
Number of reflections | 51811 | 4434 |
<I/σ(I)> | 8 | |
Completeness [%] | 99.9 | |
Redundancy | 8.6 | |
CC(1/2) | 0.997 | 0.550 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1M ADA pH 7, 34% PEG 400 and 0.3 M lithium sulfate monohydrate |