8DGR
Crystal Structure of scFv(F8) Antibody Fragment
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-03-22 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.979340 |
Spacegroup name | I 41 2 2 |
Unit cell lengths | 119.644, 119.644, 74.856 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.530 - 1.560 |
Rwork | 0.175 |
R-free | 0.19490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5b3n |
RMSD bond length | 0.012 |
RMSD bond angle | 1.736 |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0350) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.530 | 1.600 |
High resolution limit [Å] | 1.560 | 1.560 |
Number of reflections | 367580 | 2582 |
<I/σ(I)> | 10708.8 | |
Completeness [%] | 99.6 | 95.52 |
Redundancy | 19.1 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 289 | 1.8 M ammonium sulfate, 0.2 M magnesium chloride, 0.1 M HEPES, 5 % glycerol |