8DGD
Crystal Structure of GAF domain-containing protein, from Klebsiella pneumoniae
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2021-07-15 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.97856 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 70.680, 114.380, 73.890 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 46.640 - 1.900 |
R-factor | 0.1763 |
Rwork | 0.172 |
R-free | 0.21960 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1vhm |
RMSD bond angle | 13.942 |
Data reduction software | XDS |
Data scaling software | SCALEPACK |
Phasing software | MOLREP |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.950 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.059 | 0.556 |
Number of reflections | 23992 | 1754 |
<I/σ(I)> | 23.49 | 3.92 |
Completeness [%] | 100.0 | |
Redundancy | 6.92 | |
CC(1/2) | 0.990 | 0.902 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.5 | 290 | Morpheus B3: 10% w/v PEG 4000, 20% v/v glycerol, 0.03M of each halide (0.3M sodium fluoride, 0.3M sodium bromide, 0.3M sodium iodide),0.1M MES/imidazole pH6.5: 321508B3, cok0-10 |