8DFX
Crystal structure of Human BTN2A1-BTN3A1 Ectodomain Complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-10-23 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9537 |
Spacegroup name | F 2 2 2 |
Unit cell lengths | 114.630, 138.930, 336.200 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.350 - 5.550 |
R-factor | 0.2942 |
Rwork | 0.290 |
R-free | 0.33610 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4f80 BTN2A1 |
RMSD bond length | 0.007 |
RMSD bond angle | 0.910 |
Data reduction software | MOSFLM |
Data scaling software | STARANISO |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.4 (3-FEB-2022)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 47.400 | 6.620 |
High resolution limit [Å] | 5.550 | 5.550 |
Rpim | 0.086 | 0.558 |
Number of reflections | 2500 | 278 |
<I/σ(I)> | 5.3 | 1.6 |
Completeness [%] | 85.9 | 49.2 |
Redundancy | 7.4 | 7.6 |
CC(1/2) | 0.919 | 0.373 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6 | 293 | 6% PEG 6000, 0.1 M magnesium sulfate, 0.1 M HEPES |