8DFW
Crystal Structure of Human BTN2A1 in Complex With Vgamma9-Vdelta2 T Cell Receptor
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-10-23 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9537 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 112.030, 218.460, 107.930 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.720 - 2.100 |
R-factor | 0.2618 |
Rwork | 0.261 |
R-free | 0.28660 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1hxm BTN2A1 |
RMSD bond length | 0.008 |
RMSD bond angle | 1.020 |
Data reduction software | MOSFLM |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | BUSTER (2.10.4 (11-DEC-2020)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.720 | 2.140 |
High resolution limit [Å] | 2.100 | 2.100 |
Rpim | 0.034 | 0.299 |
Number of reflections | 77369 | 3834 |
<I/σ(I)> | 13.78 | 2.84 |
Completeness [%] | 100.0 | 100 |
Redundancy | 13.4 | 13.8 |
CC(1/2) | 0.998 | 0.725 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 20% PEG 3350, 0.2 M sodium malonate-malonic acid |