8DFU
Cryo-EM structure of conjugation pili from Aeropyrum pernix
EM specimen
| Vitrification | Yes |
| Staining | Yes |
| Instrument | LEICA EM GP |
| Staining material | Uranyl Acetate |
EM Data Collection
| Microscope | FEI TITAN |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K3 (6k x 4k) |
3D Reconstruction
| Method | HELICAL |
| Resolution (Å) | 3.44 |
| Software | cryoSPARC |
| CTF correction | PHASE FLIPPING ONLY |
