8DFK

X-ray crystal structure of Bacillus subtilis ComEA

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	80
Detector technology	PIXEL
Collection date	2018-11-17
Detector	DECTRIS PILATUS 6M
Wavelength(s)	0.97946
Spacegroup name	I 2 2 2
Unit cell lengths	114.525, 116.482, 183.268
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	36.010 - 3.200
R-factor	0.2562
Rwork	0.254
R-free	0.27600
Structure solution method	SAD
RMSD bond length	0.003
RMSD bond angle	0.546
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.260
High resolution limit [Å]	3.200	3.200
Number of reflections	20483	1011
<I/σ(I)>	13.15
Completeness [%]	99.5
Redundancy	13.3
CC(1/2)	0.999	0.516

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	22% PEG 500, 0.1 M succinate [pH 5.5]