8DF2

The structure of the 'ALT' construct of the Amuc_1438 glycopeptidase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-002
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-16
Detector	DECTRIS PILATUS 200K
Wavelength(s)	1.54187
Spacegroup name	P 21 21 21
Unit cell lengths	88.581, 146.094, 147.289
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	24.930 - 2.350
R-factor	0.2243
Rwork	0.222
R-free	0.26990
Structure solution method	SAD
RMSD bond length	0.005
RMSD bond angle	0.651
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.390
High resolution limit [Å]	2.350	2.350
Rpim	0.072	0.228
Number of reflections	78394	3818
<I/σ(I)>	8.2
Completeness [%]	98.2
Redundancy	3.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2M (NH4)2SO4, 20% (w/v) PEG3350 and 0.1M HEPES pH 7.5