8DF0

Abp1D receptor binding domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	100
Detector technology	CMOS
Collection date	2019-10-25
Detector	RDI CMOS_8M
Wavelength(s)	0.9762
Spacegroup name	P 42 2 2
Unit cell lengths	91.970, 91.970, 176.915
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	44.510 - 2.100
R-factor	0.2265
Rwork	0.226
R-free	0.23940
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8dez
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	44.510	2.175
High resolution limit [Å]	2.100	2.100
Rmerge	0.272	2.938
Rmeas	0.282	3.049
Rpim	0.075	0.811
Number of reflections	45137	4370
<I/σ(I)>	11.9
Completeness [%]	99.8	99.36
Redundancy	13.9	14
CC(1/2)	0.996	0.434

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		292.15	0.8 M (NH4)2SO4, 0.025 M Citric Acid pH 3.56