8DEH
Ankyrin domain of SKD3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-12 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9794 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 43.284, 61.130, 47.571 |
| Unit cell angles | 90.00, 106.26, 90.00 |
Refinement procedure
| Resolution | 45.668 - 1.815 |
| R-factor | 0.1645 |
| Rwork | 0.163 |
| R-free | 0.18940 |
| Structure solution method | SAD |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | MLPHARE |
| Refinement software | PHENIX (3.27) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.880 |
| High resolution limit [Å] | 1.815 | 1.815 |
| Rpim | 0.059 | 0.328 |
| Number of reflections | 20905 | 1015 |
| <I/σ(I)> | 9.9 | |
| Completeness [%] | 99.1 | |
| Redundancy | 4.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 287 | 50 mM EPPS pH 8.0, 35% (v/v) PEP426, and 250 mM ammonium sulfate |
