8DDK

CCHFV GP38 Hoti/Kosovo bound with CC5_17

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2021-12-11
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.0
Spacegroup name	P 43 21 2
Unit cell lengths	175.676, 175.676, 92.158
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	43.070 - 3.860
R-factor	0.3052
Rwork	0.304
R-free	0.33470
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	8dc5
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	3.920
High resolution limit [Å]	3.850	3.850
Rpim	0.100	0.480
Number of reflections	13942	1363
<I/σ(I)>	21.8
Completeness [%]	98.6
Redundancy	7.8
CC(1/2)	0.881	0.763

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	20% PEG1000, and 0.1M HEPES pH 6.4