8DCY

CCHFV GP38 Hoti/Kosovo bound with 13G8 Fab

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2021-09-30
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	P 43 21 2
Unit cell lengths	175.919, 175.919, 93.220
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	45.060 - 3.620
R-factor	0.2816
Rwork	0.281
R-free	0.29930
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6vkf
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.660
High resolution limit [Å]	3.600	9.750	3.600
Rmerge	0.163	0.055	1.741
Rmeas	0.172	0.058	1.828
Rpim	0.052	0.018	0.554
Number of reflections	17277	980	829
<I/σ(I)>	5.7
Completeness [%]	100.0	99.9	100
Redundancy	10.8	9.8	10.2
CC(1/2)		0.999	0.780

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	1.1M sodium malonate, 0.1M HEPES pH 7.0, and 0.5% Jeffamine ED-2001