8DCT
Lysozyme cluster 3 dual apo structure
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS-II BEAMLINE 17-ID-1 |
| Synchrotron site | NSLS-II |
| Beamline | 17-ID-1 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-12-14 |
| Detector | DECTRIS EIGER X 9M |
| Wavelength(s) | 0.92 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 79.230, 79.230, 37.520 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 39.650 - 2.000 |
| R-factor | 0.20917 |
| Rwork | 0.206 |
| R-free | 0.26479 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4n8z |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.420 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0267) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.120 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.360 | 1.900 |
| Number of reflections | 14274 | 2226 |
| <I/σ(I)> | 2.34 | |
| Completeness [%] | 92.4 | |
| Redundancy | 2.99 | |
| CC(1/2) | 0.869 | 0.145 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.6 | 290 | 4% SODIUM CHLORIDE, 100MM SODIUM ACETATE PH 4.6, 120MG/ML |
