8DC5

CCHFV GP38 Hoti/Kosovo

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-BM
Synchrotron site	APS
Beamline	19-BM
Temperature [K]	100
Detector technology	CCD
Collection date	2021-09-30
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	C 1 2 1
Unit cell lengths	171.633, 75.322, 62.218
Unit cell angles	90.00, 110.41, 90.00

Refinement procedure

Resolution	29.160 - 3.210
R-factor	0.2767
Rwork	0.275
R-free	0.30320
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6vkf
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.260
High resolution limit [Å]	3.200	8.670	3.200
Rmerge	0.113	0.091	0.680
Rmeas	0.134	0.113	0.794
Rpim	0.072	0.066	0.408
Total number of observations	42451
Number of reflections	11973	439	612
<I/σ(I)>	17
Completeness [%]	97.9	68.1	100
Redundancy	3.5	2.9	3.8
CC(1/2)		0.973	0.757

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293.15	20% PEG3350, 0.4M NH4I