8DB3

Crystal structure of KaiC with truncated C-terminal coiled-coil domain

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2017-11-03
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.8855
Spacegroup name	C 2 2 21
Unit cell lengths	94.060, 197.280, 150.190
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	59.750 - 2.900
R-factor	0.2397
Rwork	0.236
R-free	0.29460
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1tf7
RMSD bond length	0.005
RMSD bond angle	0.773
Data reduction software	MOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.750	3.004
High resolution limit [Å]	2.900	2.900
Number of reflections	31367	3103
<I/σ(I)>	5.54	1.15
Completeness [%]	99.9
Redundancy	7.2
CC(1/2)	0.976	0.343

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	0.2 M Ammonium acetate, 0.1 M Sodium citrate tribasic dihydrate pH 5.6, 30% w/v Polyethylene glycol 4,000