8DAK
Crystal structure of the GDP-D-glycero-4-keto-d-lyxo-heptose-3-epimerase from Campylobacter jejuni, serotype HS:3
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SEALED TUBE |
| Source details | BRUKER D8 QUEST |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2021-07-20 |
| Detector | Bruker PHOTON II |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 61.534, 67.932, 96.125 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.100 - 1.500 |
| R-factor | 0.1932 |
| Rwork | 0.192 |
| R-free | 0.21880 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 7m14 |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.596 |
| Data reduction software | SAINT |
| Data scaling software | SADABS |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0253) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.600 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Number of reflections | 63904 | 10456 |
| <I/σ(I)> | 11.4 | 2.8 |
| Completeness [%] | 97.5 | 89.6 |
| Redundancy | 5.7 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 10-12% PEG8000, 0.75 M tetramethylammonium chloride, 100 mM homopiperazine-1,4-bis(2-ethanesulfonic acid), pH 5.0, protein pre-incubated with 5 mM GDP |
