8D9O
De Novo Photosynthetic Reaction Center Protein in Apo-State
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 HF |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-12-06 |
| Detector | RIGAKU SATURN 944+ |
| Wavelength(s) | 1.54178 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 89.692, 23.693, 72.756 |
| Unit cell angles | 90.00, 99.93, 90.00 |
Refinement procedure
| Resolution | 18.460 - 1.780 |
| R-factor | 0.1841 |
| Rwork | 0.183 |
| R-free | 0.20860 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5vjs |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.782 |
| Data reduction software | XDS |
| Data scaling software | XSCALE (VERSION July 4, 2012 BUILT=20130706) |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 18.460 | 1.829 |
| High resolution limit [Å] | 1.780 | 1.783 |
| Rmerge | 0.047 | 0.175 |
| Number of reflections | 14128 | 737 |
| <I/σ(I)> | 17.12 | 3.79 |
| Completeness [%] | 95.1 | 68.9 |
| Redundancy | 3.4 | |
| CC(1/2) | 0.950 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 4.8 | 277 | 24% w/v PEG1500, 100 mM CdCl2, 100 mM Na acetate, pH 4.8. Cryoprotectant: 2-methyl-2.4-pentanediol |
