8D7P

Human Casein kinase 1 delta in complex with phosphorylated Drosophila PERIOD peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-12-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.03
Spacegroup name	P 1
Unit cell lengths	48.780, 56.740, 65.940
Unit cell angles	108.93, 95.63, 108.69

Refinement procedure

Resolution	48.514 - 2.250
R-factor	0.199
Rwork	0.196
R-free	0.25110
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6pxo
RMSD bond length	0.009
RMSD bond angle	1.069
Data reduction software	iMOSFLM
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX ((1.15.2_3472))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	60.770	2.320
High resolution limit [Å]	2.250	2.250
Rmerge	0.116	0.312
Number of reflections	27457	2505
<I/σ(I)>	5.5	2.7
Completeness [%]	93.7
Redundancy	3.1
CC(1/2)	0.980	0.850

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.5	295	0.16M Succinic Acid (5.5) 23% PEG3350