8D7D

Crystal structure of hen egg white lysozyme at 225 Kelvin (Triplicate)

Experimental procedure

Experimental method	LAUE
Source type	ROTATING ANODE
Source details	BRUKER TURBO X-RAY SOURCE
Temperature [K]	225
Detector technology	CMOS
Collection date	2020-11-04
Detector	BRUKER PHOTON 100
Wavelength(s)	1.54184
Spacegroup name	P 43 21 2
Unit cell lengths	79.487, 79.487, 37.495
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	22.498 - 1.500
Rwork	0.171
R-free	0.20710
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3a8z
RMSD bond length	0.013
RMSD bond angle	1.838
Data reduction software	SAINT
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	22.500	22.500
High resolution limit [Å]	1.500	1.500
Number of reflections	19769	18747
<I/σ(I)>	5.87
Completeness [%]	99.6
Redundancy	1.89
CC(1/2)	0.827	0.827

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	4.6	295	1.2 M NaCl and 100 mM AcONa pH 4.6