8D6O
Nanorana parkeri saxiphilin:F-STX (soaked)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-B |
Synchrotron site | APS |
Beamline | 23-ID-B |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-12-10 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.033167 |
Spacegroup name | H 3 |
Unit cell lengths | 229.186, 229.186, 67.347 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 43.310 - 2.200 |
R-factor | 0.199 |
Rwork | 0.197 |
R-free | 0.24310 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 8d6g |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 43.310 | 2.279 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.071 | |
Number of reflections | 66898 | 6682 |
<I/σ(I)> | 19.64 | 1.35 |
Completeness [%] | 99.9 | |
Redundancy | 10.6 | |
CC(1/2) | 0.999 | 0.602 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 277.15 | 20-25% v/v PEG400, 4-5% w/v PGA-LM, 100-200 mM sodium acetate, pH 5.0, 1 mM (final concentration) F-STX was added during soaking |