8D5K

The complex of Pre-mRNA-Processing Factor 19 (Prpf19) peptide KYLQVASHV Presented by H2-Kd

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-E
Synchrotron site	APS
Beamline	21-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-06-10
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98
Spacegroup name	C 1 2 1
Unit cell lengths	115.370, 66.200, 56.790
Unit cell angles	90.00, 92.42, 90.00

Refinement procedure

Resolution	41.349 - 2.066
Rwork	0.203
R-free	0.23350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vgk
RMSD bond length	0.007
RMSD bond angle	1.874
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.8.0350)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	41.349	2.000
High resolution limit [Å]	1.950	1.950
Rmerge	0.069	0.281
Rmeas	0.096	0.396
Rpim	0.067	0.278
Number of reflections	23784	8577
<I/σ(I)>	14.6
Completeness [%]	90.4	97
Redundancy	2.3	1.9
CC(1/2)	0.993	0.899

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277.15	10% w/v PEG8000, 200 mM magnesium acetate