8D5F

The complex of Gtf2b neoantigen TGAARFDEF Presented by H2-Dd

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-E
Synchrotron site	APS
Beamline	21-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-02
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	52.300, 68.860, 109.440
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.233 - 2.310
Rwork	0.193
R-free	0.19910
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	{DB entry 5KD7
RMSD bond length	0.011
RMSD bond angle	1.979
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.8.0350)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.233	2.390
High resolution limit [Å]	2.310	2.310
Rmerge	0.120	0.046
Rmeas	0.143	0.054
Rpim	0.077	0.027
Number of reflections	17756	17756
<I/σ(I)>	10.7
Completeness [%]	99.2
Redundancy	6	6
CC(1/2)	0.993	0.996

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.3	309.15	20% PEG3350, 200 nM sodium tartrate dibasic dihydrate