8D5E

The complex of Gtf2b Peptide TGAASFDEF Presented by H2-Dd

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-02
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	53.750, 80.590, 100.020
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.391 - 2.460
Rwork	0.199
R-free	0.24160
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5kd7
RMSD bond length	0.006
RMSD bond angle	1.417
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	REFMAC (5.8.0350)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	47.391	47.350	2.560
High resolution limit [Å]	2.460	8.870	2.460
Rmerge	0.190	0.077	0.813
Rmeas	0.208	0.084	0.895
Rpim	0.084	0.034	0.368
Total number of observations		2304	10352
Number of reflections	16346	401	1801
<I/σ(I)>	10.7	27.2	2.7
Completeness [%]	99.9	99.7	99.5
Redundancy	6	5.7	5.7
CC(1/2)	0.988	0.990	0.782

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		309.15	15% w/v PEG1500