8D5E
The complex of Gtf2b Peptide TGAASFDEF Presented by H2-Dd
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-04-02 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.98 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 53.750, 80.590, 100.020 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.391 - 2.460 |
Rwork | 0.199 |
R-free | 0.24160 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5kd7 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.417 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | REFMAC (5.8.0350) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 47.391 | 47.350 | 2.560 |
High resolution limit [Å] | 2.460 | 8.870 | 2.460 |
Rmerge | 0.190 | 0.077 | 0.813 |
Rmeas | 0.208 | 0.084 | 0.895 |
Rpim | 0.084 | 0.034 | 0.368 |
Total number of observations | 2304 | 10352 | |
Number of reflections | 16346 | 401 | 1801 |
<I/σ(I)> | 10.7 | 27.2 | 2.7 |
Completeness [%] | 99.9 | 99.7 | 99.5 |
Redundancy | 6 | 5.7 | 5.7 |
CC(1/2) | 0.988 | 0.990 | 0.782 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 309.15 | 15% w/v PEG1500 |