8D53

Crystal Structure of Mosaic HIV-1 Envelope (MosM3.3) in Complex with antibodies PGT124 and 35O22 at 3.25 Angstrom

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 23-ID-D
Synchrotron site	APS
Beamline	23-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-07-10
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.03316
Spacegroup name	P 3 1 2
Unit cell lengths	139.266, 139.266, 320.332
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	42.790 - 3.240
R-factor	0.3176
Rwork	0.317
R-free	0.33080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5cez
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.19.1_4122)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.310
High resolution limit [Å]	3.240	8.810	3.250
Rmerge	0.157	0.058	1.285
Rmeas	0.173	0.065	1.386
Rpim	0.070	0.028	0.505
Number of reflections	47686	2947	1118
<I/σ(I)>	5.3
Completeness [%]	83.6	98.2	39.8
Redundancy	5	4.8	5.9
CC(1/2)		0.995	0.621

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	1.825M ammonium sulfate, 2% PGE 400, 0.1M HEPES, pH 7.1