8D4Y
C-terminal SANT-SLIDE domain of human Chromodomain-helicase-DNA-binding protein 4 (CHD4)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-11-24 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9537 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 65.432, 66.665, 99.370 |
Unit cell angles | 90.00, 100.77, 90.00 |
Refinement procedure
Resolution | 19.900 - 2.900 |
R-factor | 0.265 |
Rwork | 0.263 |
R-free | 0.30120 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Alphafold |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | Auto-Rickshaw |
Refinement software | PHENIX (1.19.2_4158) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 48.809 | 48.809 | 3.070 |
High resolution limit [Å] | 2.900 | 8.570 | 2.900 |
Rmerge | 0.116 | 0.065 | 0.811 |
Rmeas | 0.131 | 0.074 | 0.916 |
Total number of observations | 82638 | ||
Number of reflections | 18602 | 764 | 2791 |
<I/σ(I)> | 9.28 | 20.23 | 1.71 |
Completeness [%] | 98.0 | 98.2 | 91.7 |
Redundancy | 4.442 | 4.113 | 4.569 |
CC(1/2) | 0.994 | 0.992 | 0.706 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 8.3 | 277.15 | 0.1 M bicine/Trizma base pH 8.3, 10% w/v PEG 8000, 20% v/v ethylene glycol, 0.1 M mixture of carboxylic acids*, and 20 mM spermidine *Sodium Formate; Ammonium acetate; Sodium citrate tribasic dihydrate; Potassium sodium tartrate tetrahydrate; Sodium oxamate |