8D4Y

C-terminal SANT-SLIDE domain of human Chromodomain-helicase-DNA-binding protein 4 (CHD4)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-11-24
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	P 1 21 1
Unit cell lengths	65.432, 66.665, 99.370
Unit cell angles	90.00, 100.77, 90.00

Refinement procedure

Resolution	19.900 - 2.900
R-factor	0.265
Rwork	0.263
R-free	0.30120
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Alphafold
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	Auto-Rickshaw
Refinement software	PHENIX (1.19.2_4158)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	48.809	48.809	3.070
High resolution limit [Å]	2.900	8.570	2.900
Rmerge	0.116	0.065	0.811
Rmeas	0.131	0.074	0.916
Total number of observations	82638
Number of reflections	18602	764	2791
<I/σ(I)>	9.28	20.23	1.71
Completeness [%]	98.0	98.2	91.7
Redundancy	4.442	4.113	4.569
CC(1/2)	0.994	0.992	0.706

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.3	277.15	0.1 M bicine/Trizma base pH 8.3, 10% w/v PEG 8000, 20% v/v ethylene glycol, 0.1 M mixture of carboxylic acids*, and 20 mM spermidine *Sodium Formate; Ammonium acetate; Sodium citrate tribasic dihydrate; Potassium sodium tartrate tetrahydrate; Sodium oxamate