8D3T
Crystal structure of GalS1 from Populus trichocarpas
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.2 |
Synchrotron site | ALS |
Beamline | 8.2.2 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-09-26 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.000 |
Spacegroup name | P 1 |
Unit cell lengths | 70.398, 83.422, 94.689 |
Unit cell angles | 76.16, 69.67, 88.57 |
Refinement procedure
Resolution | 80.840 - 2.370 |
R-factor | 0.1976 |
Rwork | 0.195 |
R-free | 0.25210 |
Structure solution method | SAD |
RMSD bond length | 0.003 |
RMSD bond angle | 0.572 |
Data reduction software | DIALS |
Data scaling software | xia2 |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 80.840 | 2.450 |
High resolution limit [Å] | 2.370 | 2.370 |
Rmerge | 0.103 | 1.036 |
Number of reflections | 78131 | 7586 |
<I/σ(I)> | 7.18 | |
Completeness [%] | 98.2 | |
Redundancy | 2.6 | |
CC(1/2) | 0.989 | 0.506 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 0.1 M Sodium citrate tribasic dihydrate pH 5.0, 10 % PEG 6,000 |