8D30

Crystal structure of the human COPB2 WD-domains

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-E
Synchrotron site	APS
Beamline	24-ID-E
Temperature [K]	100
Detector technology	PIXEL
Collection date	2020-11-08
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.97918
Spacegroup name	P 21 21 21
Unit cell lengths	67.074, 134.062, 144.270
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.120 - 2.400
R-factor	0.221
Rwork	0.219
R-free	0.24700
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5nzr
RMSD bond length	0.010
RMSD bond angle	1.190
Data reduction software	HKL-3000
Data scaling software	SCALEPACK
Phasing software	PHASER
Refinement software	BUSTER (2.10.3)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.440
High resolution limit [Å]	2.400	6.510	2.400
Rmerge	0.080	0.039	1.005
Rmeas	0.089	0.043	1.123
Rpim	0.037	0.018	0.486
Total number of observations	288205
Number of reflections	51291	2707	2439
<I/σ(I)>	8.9
Completeness [%]	99.0	96.2	97.1
Redundancy	5.6	5.4	4.8
CC(1/2)		0.997	0.496

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		291	20%(w/v) PEG 3350, 0.05M Magnesium Formate