8D2Y

Y430F mutant of D-ornithine/D-lysine decarboxylase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-02-27
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.0000
Spacegroup name	C 1 2 1
Unit cell lengths	139.740, 50.740, 73.320
Unit cell angles	90.00, 120.80, 90.00

Refinement procedure

Resolution	36.660 - 1.220
R-factor	0.1344
Rwork	0.134
R-free	0.15180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6n2h
RMSD bond length	0.008
RMSD bond angle	1.145
Data reduction software	autoPROC
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	36.660	1.265
High resolution limit [Å]	1.220	1.220
Number of reflections	101867	628
<I/σ(I)>	17.78
Completeness [%]	77.9
Redundancy	13.8
CC(1/2)	0.999	0.580

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	293	0.1 M HEPES, pH 7.5, 0.2-0.4 M NaOAc, 20% PEG 4000