8D2V
Zebrafish MFSD2A isoform B in inward open ligand 1B conformation
EM specimen
Vitrification | Yes |
Instrument | LEICA EM GP |
EM Data Collection
Microscope | TFS KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 0.01 |
Energy Filter | GIF Bioquantum |
Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 4.1 |
Software | cryoSPARC,Coot,PHENIX,UCSF Chimera,SerialEM |
CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |