8D1N
bBest2_345 Ca2+-bound open state
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
EM Data Collection
Microscope | TFS KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Cs (mm) | 2.7 |
Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 1.93 |
Number of Particle | 1070653 |
Software | cryoSPARC,Coot,REFMAC,Leginon,PHENIX |
Symmetry | C5 |
CTF correction | NONE |