8D0V

Human LanCL1 C264A mutant bound to GSH

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2021-03-01
Detector	DECTRIS EIGER X 9M
Wavelength(s)	1.12723
Spacegroup name	P 21 21 21
Unit cell lengths	55.587, 120.257, 143.019
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 1.790
R-factor	0.18247
Rwork	0.181
R-free	0.20186
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3e6u
RMSD bond length	0.005
RMSD bond angle	1.215
Data reduction software	AutoProcess
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	REFMAC (5.8.0267)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	92.043	1.792
High resolution limit [Å]	1.786	1.786
Rmerge	0.099	1.083
Rmeas	0.105	1.158
Rpim	0.034	0.393
Number of reflections	90992	858
<I/σ(I)>	13.5	2.1
Completeness [%]	99.3	93.7
Redundancy	9.5	8.2
CC(1/2)	0.999	0.879

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		282.15	0.70-0.75 M succinic acid pH 7.0, 0.1 M bis-tris-propane pH 7.0.