8CZH
Human BAK in complex with the dM2 peptide
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-08-07 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.97918 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 102.130, 41.040, 47.020 |
Unit cell angles | 90.00, 93.64, 90.00 |
Refinement procedure
Resolution | 46.920 - 1.300 |
R-factor | 0.1795 |
Rwork | 0.178 |
R-free | 0.20050 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5vx0 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.20.1_4487) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.920 | 46.920 | 1.330 |
High resolution limit [Å] | 1.300 | 5.810 | 1.300 |
Rmerge | 0.040 | 0.025 | 1.714 |
Rmeas | 0.043 | 0.027 | 1.854 |
Total number of observations | 320911 | ||
Number of reflections | 46424 | 571 | 3181 |
<I/σ(I)> | 17.3 | 67.22 | 1.29 |
Completeness [%] | 96.8 | 99 | 90.8 |
Redundancy | 6.913 | 6.573 | 6.854 |
CC(1/2) | 1.000 | 1.000 | 0.704 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 3.5 M sodium formate |