8CYL
Ast89 P domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE BM30A |
Synchrotron site | ESRF |
Beamline | BM30A |
Temperature [K] | 291.15 |
Detector technology | PIXEL |
Collection date | 2017-11-10 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 1.00 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 74.590, 78.410, 100.860 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 42.415 - 1.888 |
R-factor | 0.1644 |
Rwork | 0.163 |
R-free | 0.19490 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 4x1w |
RMSD bond length | 0.004 |
RMSD bond angle | 0.770 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.13_2998: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 42.425 | 42.425 |
High resolution limit [Å] | 1.888 | 1.888 |
Number of reflections | 47320 | 47320 |
<I/σ(I)> | 9.19 | |
Completeness [%] | 98.2 | |
Redundancy | 1.36 | |
CC(1/2) | 0.903 | 0.903 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 291.15 | 0.1 M citric acid (pH 4) and 30% PEG6000 |