8CYK

Crystal structure of hallucinated protein HALC1_878

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.1
Synchrotron site	ALS
Beamline	8.2.1
Temperature [K]	100
Detector technology	CCD
Collection date	2022-04-07
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.00003
Spacegroup name	I 1 2 1
Unit cell lengths	64.174, 55.453, 82.807
Unit cell angles	90.00, 94.00, 90.00

Refinement procedure

Resolution	48.980 - 1.650
R-factor	0.1864
Rwork	0.185
R-free	0.21680
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	Design model
RMSD bond length	0.011
RMSD bond angle	1.124
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.980	1.709
High resolution limit [Å]	1.650	1.650
Number of reflections	34565	3322
<I/σ(I)>	15.91	3.96
Completeness [%]	97.2	95.7
Redundancy	2	2
CC(1/2)	0.999	0.942

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	3.5	293	0.1 M citric acid 3M NaCl