8CY5

CamA Adenine Methyltransferase Complexed to Cognate Substrate DNA and Compound 39

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2022-03-26
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	81.550, 161.231, 230.294
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	47.030 - 2.500
R-factor	0.1731
Rwork	0.172
R-free	0.20910
Structure solution method	FOURIER SYNTHESIS
Starting model (for MR)	7lnj
RMSD bond length	0.002
RMSD bond angle	0.498
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.030	2.580
High resolution limit [Å]	2.500	2.500
Rmerge	0.244
Rpim	0.057	0.642
Number of reflections	105385	9778
<I/σ(I)>	12.7
Completeness [%]	99.3
Redundancy	12.6
CC(1/2)	0.990	0.431

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		292	21-24% PEG 3350, 0.1 M Tris-HCl pH 7-7.5, 0.28M Potassium Citrate