8CWR
Complex structure of WhiB3 and the SigmaAr4-RNAP Beta flap tip chimera in space group R3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SSRL BEAMLINE BL12-2 |
Synchrotron site | SSRL |
Beamline | BL12-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2021-02-11 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 0.9795 |
Spacegroup name | H 3 |
Unit cell lengths | 120.809, 120.809, 31.758 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.870 - 1.500 |
R-factor | 0.1843 |
Rwork | 0.183 |
R-free | 0.21220 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 8cwt |
RMSD bond length | 0.011 |
RMSD bond angle | 1.290 |
Data reduction software | HKL-3000 (v1.0) |
Data scaling software | HKL-3000 (v1.0) |
Phasing software | AutoSol (v1.0) |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.530 |
High resolution limit [Å] | 1.500 | 1.500 |
Rmerge | 0.077 | 2.160 |
Rmeas | 0.079 | 2.239 |
Rpim | 0.018 | 0.578 |
Number of reflections | 27421 | 2696 |
<I/σ(I)> | 45.4 | 1.6 |
Completeness [%] | 99.8 | 99.7 |
Redundancy | 18.1 | 14.2 |
CC(1/2) | 1.000 | 0.713 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION | 8 | 293 | 10-20 mM NiCl2, 0.8-1.0 M Li2SO4 |