8CUJ
Human excitatory amino acid transporter 3 (EAAT3) protomer in an outward facing apo state in 150 mM NMDG-Cl
EM specimen
| Vitrification | Yes |
| Instrument | FEI VITROBOT MARK IV |
EM Data Collection
| Microscope | FEI TITAN KRIOS |
| Electron Gun | FIELD EMISSION GUN |
| Accelerating Voltage (kV) | 300 |
| Detector | GATAN K3 BIOQUANTUM (6k x 4k) |
3D Reconstruction
| Method | SINGLE PARTICLE |
| Resolution (Å) | 3.04 |
| Number of Particle | 2553613 |
| Software | RELION,PHENIX,cryoSPARC,Leginon,CTFFIND,UCSF ChimeraX,Coot |
| Symmetry | C1 |
| CTF correction | PHASE FLIPPING AND AMPLITUDE CORRECTION |
