8CTY

12-mer DNA structure of ExBIM bound to RNase-H

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-G
Synchrotron site	APS
Beamline	21-ID-G
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2018-04-10
Detector	MAR scanner 300 mm plate
Wavelength(s)	0.97856
Spacegroup name	P 1
Unit cell lengths	64.976, 65.834, 95.723
Unit cell angles	84.54, 88.20, 62.49

Refinement procedure

Resolution	32.730 - 2.300
R-factor	0.1989
Rwork	0.196
R-free	0.26060
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3ey1
RMSD bond length	0.009
RMSD bond angle	1.002
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX (1.20.1_4487)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	32.730	2.380
High resolution limit [Å]	2.300	2.300
Rmerge	0.133	1.043
Rpim	0.053	0.683
Number of reflections	61160	5899
<I/σ(I)>	3.8	0.8
Completeness [%]	98.1	94.4
Redundancy	3.8	3.1
CC(1/2)		0.386

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	0.1 M HEPES sodium pH = 7.5, 10% v/v 2-propanol and 20% w/v PEG 4000