8CRZ
Crystal structure of alien DNA CTSZZPBSBSZPPBAG in a tetragonal lattice
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 31-ID |
Synchrotron site | APS |
Beamline | 31-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2020-07-22 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 0.97933 |
Spacegroup name | P 42 21 2 |
Unit cell lengths | 46.496, 46.496, 42.452 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.794 - 1.200 |
R-factor | 0.1854 |
Rwork | 0.184 |
R-free | 0.20000 |
Structure solution method | MOLECULAR REPLACEMENT |
Data reduction software | XDS |
Data scaling software | Aimless (0.7.4) |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 46.500 | 46.500 | 1.220 |
High resolution limit [Å] | 1.200 | 6.570 | 1.200 |
Rmerge | 0.048 | 0.051 | 0.209 |
Rmeas | 0.050 | 0.055 | 0.217 |
Rpim | 0.015 | 0.019 | 0.060 |
Total number of observations | 181739 | 1002 | 9464 |
Number of reflections | 15095 | 122 | 739 |
<I/σ(I)> | 34.2 | 42.6 | 13.1 |
Completeness [%] | 99.8 | 96.9 | 99.1 |
Redundancy | 12 | 8.2 | 12.8 |
CC(1/2) | 0.998 | 0.996 | 0.993 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 293 | 0.05 M HEPES, pH 7.0, 2.0 M ammonium sulfate |