8CQX
Ribokinase from T.sp mutant A92G
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID23-1 |
Synchrotron site | ESRF |
Beamline | ID23-1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2022-09-03 |
Detector | DECTRIS EIGER R 1M |
Wavelength(s) | 0.8856 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 44.307, 155.608, 82.915 |
Unit cell angles | 90.00, 96.37, 90.00 |
Refinement procedure
Resolution | 29.520 - 2.270 |
R-factor | 0.23322 |
Rwork | 0.229 |
R-free | 0.31148 |
Structure solution method | MOLECULAR REPLACEMENT |
RMSD bond length | 0.009 |
RMSD bond angle | 1.670 |
Data reduction software | iMOSFLM |
Data scaling software | SCALA |
Phasing software | PHASER |
Refinement software | REFMAC (5) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 30.000 | 2.390 |
High resolution limit [Å] | 2.270 | 2.270 |
Rmerge | 0.133 | 0.340 |
Rmeas | 0.158 | |
Number of reflections | 51152 | 25537 |
<I/σ(I)> | 3.886 | 2.03 |
Completeness [%] | 99.4 | |
Redundancy | 3.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20% peg3350, 0,45 M NaCl, 0,1 M citric acid pH 5.0, 5 mM MgCl2, 5 mM ADP |